Kayayyaki

Hanyoyin ƙwayoyin cuta na ƙwayoyin cuta a cikin cututtukan hanta na cirrhotic irin su ciwon hanta na hepatitis C (HCV), hepatitis autoimmune (AIH) da kuma biliary cirrhosis (PBC) ba su da kyau.Kwayoyin da aka bayyana daban-daban sau da yawa suna da mahimmanci a cikin cututtukan cututtuka.Haɓaka haɓakawa na ragewa (SSH) hanya ce mai faɗin kwayar halitta wacce ke wadatar da kwafin mRNA daban-daban.Mun yi niyya don yin sabbin abubuwan lura game da bambancin jinsi a cikin cirrhosis ta amfani da SSH haɗe tare da ƙimar juzu'in juzu'i na ainihin-lokaci polymerase chain reaction (RT-PCR).SSH ya bincika rubutun hanta a cikin HCV cirrhosis, AIH cirrhosis, PBC, da kuma hanta mara lafiya.Sakamakon ƙarin DNA (cDNA) clones an sake duba su don bambancin magana ta ɗigo-blot hybridization sannan aka jera su.An ƙididdige maganganun jinsin da aka zaɓa ta hanyar RT-PCR na ainihi.Bayan SSH, an sake duba clones 694 don bayyana bambancin jinsi, wanda 145 aka jera su kuma an samo su daga 89 daban-daban kwayoyin.clones bakwai sun kasance masu kamanceceniya ne kawai tare da jerin jerin alamomin da aka bayyana (EST) da ke ɓoye kwayoyin halitta waɗanda basu da sananniyar aiki.An tabbatar da bayanin da aka tsara na kwayoyin halitta guda hudu ta hanyar RT-PCR na ainihi: transmembrane 4 superfamily memba 3 (tetraspanin CO-029) a cikin kowane nau'i na cirrhosis, furotin da ke hulɗa da shinge (HIP) a cikin AIH cirrhosis da chitinase 3-like-1 (HC gp-39 ko ykl-40) da arginine-glutamic acid maimaita (RERE) a cikin HCV cirrhosis.RERE gene polymorphisms da bambance-bambancen rabe-rabe an lura dasu a duk kyallen da aka bincika.Tetraspanin CO-029 up-regulation da farko an keɓance shi zuwa ƙwayoyin bile ductular.A ƙarshe, an yi nazarin abubuwan lura na banbance-banbancen jinsi a cikin cirrhosis na ɗan adam ta amfani da SSH a matsayin kayan aikin ganowa na farko.Musamman, ƙarin nazarin kwayoyin RERE da samfuransa a cikin cututtukan hanta masu alaƙa da HCV suna da garanti.