Kayayyaki

Cellulose shine mafi yawan adadin polymer da ake sabuntawa akan Duniya kuma babban sashi na bangon tantanin halitta.A cikin shuke-shuke na jijiyoyin jini, ƙwayar cellulose yana haɓaka ta hanyar babban, plasma membrane-localized cellulose synthase complex (CSC), wanda aka gani a matsayin tsarin rosette hexameric.Ana buƙatar isoforms guda uku na musamman na cellulose synthase (CESA) don taron CSC da aiki.Koyaya, bayanin ko dai lamba ko stoichiometry na CESAs a cikin CSC ya kasance da wuya.A cikin wannan binciken, mun nuna 1: 1: 1 stoichiometry tsakanin Arabidopsis thaliana secondary cell isozymes: CESA4, CESA7, da CESA8.An ƙaddara wannan rabo ta amfani da hanya mai sauƙi amma kyakkyawar hanyar ƙididdige immunoblotting ta amfani da ƙayyadaddun ƙwayoyin rigakafi da kuma (35) S-lakabin ma'aunin furotin na kowane CESA.Bugu da ƙari, an gano stoichiometry da aka lura yana daidaitawa tare da axis na tushe, wanda ke wakiltar haɓakar haɓakawa.Sakamakonmu ya cika nazarce-nazarce na kwanan nan wanda ke nuni zuwa ga microfibril cellulose mai sarkar 18.A dunƙule, muna ba da shawarar cewa CSC ta ƙunshi hexamer na masu daidaitawar CESA mai ƙarfi, tare da kowane CESA a daidai gwargwado.Wannan binciken shine ci gaba mai mahimmanci don fahimtar yadda CESAs ke haɗawa don samar da manyan ka'idodin tsari, wanda shine maɓalli mai mahimmanci na microfibril cellulose da kaddarorin bangon tantanin halitta.