Kayayyaki

Tasirin da ba zato ba tsammani na wasu nau'o'in maganin ciwon zuciya shine osteoporosis wanda zai iya zama, aƙalla a wani ɓangare, yana da alaƙa da ɓarnawar ƙwayar ƙwayar cuta (MSC).Manufar binciken na yanzu shine kwatanta tasirin fondaparinux (FDP), wani labari na antithrombotic tare da al'ada da aka yi amfani da shi na heparin maras nauyi, tinzaparin (TZP) akan yaduwar MSC da bambanci.MSCs an ware su daga kasusuwa na trabecular na 14 marasa lafiya masu rauni ta hanyar tsarin narkewa na tushen collagenase da kuma fadada a cikin daidaitattun yanayi har zuwa nassi na 3. Yaduwa da bambance-bambancen MSC zuwa chondrocytes da osteoblasts an kiyasta tare da ko ba tare da ƙarin FDP da TZP ta amfani da daidaitattun in vitro. gwaje-gwaje da kuma yawan adadin magunguna.An yi amfani da cytometry mai gudana don ƙirar MSC.A cikin shekarun da aka yi nazari (shekaru 17-74) mitar MSC a cikin sassan da aka fitar da collagenase shine sel 641/10 (6) (kewayon 110-2,158) kuma halayen haɓakarsu sun kasance ~ 4 kwanaki / yawan jama'a.Al'adu suna da ma'auni na MSC phenotype (CD73+, CD105+, CD146+, CD106+, da CD166+).An ƙididdige yaduwar kwayar cutar ta hanyar duka-ƙarshen-fibroblast mai mulkin mallaka (CFU-F) da tetrazolium gishiri mai launi XTT.A cikin gwaje-gwajen guda biyu, an hana haɓakar MSC ta hanyar ƙari na TZP, musamman a babban taro.Sabanin haka, FDP ba ta da wani tasiri akan yaduwar MSC.Bambance-bambancen osteogenic da bambance-bambancen chondrogenic ba su shafi ƙari na ko dai TZP ko FDP ba.Duk da yake yaduwar MSC, amma ba bambance-bambance ba, TZP ya yi mummunan tasiri, babu wata shaida game da illar FDP a cikin wannan tsarin samfurin in vitro wanda yayi jayayya da kyau don amfani da shi a cikin tsarin orthopedic.