Kayayyaki

Mun ƙirƙira sabon tsarin isar da ƙaramin tsoma baki na RNA (siRNA) ta amfani da hadaddun ternary tare da polyethyleneimine (PEI) da γ-polyglutamic acid (γ-PGA), wanda ya nuna tasirin shiru kuma babu cytotoxicity.Rukunin binary na siRNA tare da PEI sun kasance kusan 73-102 nm a girman barbashi da 45-52 mV a cikin ζ-mai yiwuwa.Sakamakon shiru na siRNA/PEI complexes ya karu tare da haɓakar PEI, kuma siRNA/PEI complexes tare da ƙimar caji fiye da 16 sun nuna alamar luciferase mai mahimmanci a cikin layin kwayar cutar kansar linzamin kwamfuta a kai a kai yana bayyana luciferase (Colon26 / Luc Kwayoyin).Koyaya, an lura da cytotoxicity mai ƙarfi da agglutination na jini a cikin hadaddun siRNA/Lipofectamine da hadaddun siRNA/PEI16.Ana yin cajin rukunin cationic tare da fili na anionic an ruwaito cewa hanya ce mai ban sha'awa don shawo kan waɗannan guba.Don haka mun shirya rukunin sirna na ternary tare da PEI (darajar caji 16) ta ƙari na γ-PGA don rage cytotoxicity da isar da siRNA.Kamar yadda aka zata, cytotoxicity na ternary complexes ya ragu tare da haɓaka abun ciki na γ-PGA, wanda ya rage yuwuwar ζ na rukunin.An gano tasirin shiru mai ƙarfi mai kwatankwacin siRNA/Lipofectamine a cikin rukunan da suka haɗa da γ-PGA tare da cajin saman anionic.An tabbatar da babban haɗe-haɗe na ternary complexes cikin ƙwayoyin Colon26/Luc tare da microcopy mai haske.Bayan da aka samu ƙwanƙwasa ƙwayar ƙwayar cuta ta waje, ikon hadaddun don yin sulhu da ƙwanƙwasa wani nau'in tsarin kula da gida, glyceraldehyde 3-phosphate dehydrogenase (GAPDH), an tantance shi a cikin ƙwayoyin B16-F10.Hadaddiyar ternary ( hadaddun siRNA/PEI16/γ-PGA12) ya nuna mahimmin tasirin ƙwanƙwasa GAPDH.Don haka, mun haɓaka tsarin isar da siRNA mai amfani.