Kayayyaki

Ana amfani da tsufa na zamani na ƙwayoyin yisti a matsayin abin koyi don tsufa na ƙwayoyin jikin mutum bayan mitotic.Yisti Saccharomyces cerevisiae da aka girma akan glucose a gaban ammonium sulphate ana amfani da shi a cikin binciken tsufa na yisti.Mun yi nazari na zamani tsufa na yisti Hansenula polymorpha girma a yanayin da ke buƙatar farko na peroxisome metabolism don girma.The chronological lifespan na H. polymorpha yana da karfi da inganta lokacin da kwayoyin girma a kan methanol ko ethanol, metabolized ta peroxisome enzymes, dangane da girma a kan glucose. wanda baya buƙatar peroxisomes.Tsawon ɗan gajeren lokaci na H. polymorpha akan glucose shine yafi saboda matsakaicin acidification, yayin da mai yiwuwa ROS ba ya taka muhimmiyar rawa.Girman sel akan methanol/methylamine maimakon methanol/ammonium sulfate ya haifar da ƙarin haɓaka rayuwa.Wannan bai da alaƙa da matsakaicin acidification.Mun nuna cewa hadawan abu da iskar shaka na methylamine ta peroxisomal amine oxidase a yanayin yunwar carbon yana da alhakin haɓaka tsawon rayuwa.The methylamine hadawan abu da iskar shaka samfurin formaldehyde an kara oxidized sakamakon a cikin NADH tsara, wanda ke taimakawa wajen ƙara ATP tsara da kuma rage ROS matakan a tsaye phase.Mun kammala cewa primary peroxisome metabolism inganta chronological lifespan na H. polymorpha.Haka kuma, yuwuwar samar da NADH a yanayin yunwar carbon ta hanyar tushen nitrogen na kwayoyin yana tallafawa ƙarin tsawon rayuwar tantanin halitta.Sakamakon haka, fassarar nazarin CLS a cikin yisti ya kamata ya haɗa da yiwuwar tasiri akan matsayin makamashi na tantanin halitta.