Kayayyaki

Hepatotoxicity na masu neman magani yana ɗaya daga cikin manyan abubuwan da ke damuwa a cikin binciken magunguna a farkon gano magunguna.Gano danniya mai oxidative na hanta na iya zama alamar farkon alamar hepatotoxicity da fa'idodin zaɓin ƙwayoyi.glutathione (GSH) da glutathione disulfide (GSSG), a matsayin daya daga cikin manyan redox redox na ciki da ke daidaita ma'aurata, suna taka muhimmiyar rawa wajen kare sel daga danniya mai oxidative wanda ke haifar da rashin daidaituwa tsakanin prooxidants da antioxidants.An yi amfani da ƙayyadaddun ƙayyadaddun ƙididdiga na ƙimar GSSG/GSH da ƙididdiga na GSH da GSSG don nuna damuwa na oxidative a cikin sel da kyallen takarda.A cikin wannan binciken, mun gwada yiwuwar yin amfani da ma'auni na GSSG/GSH na biliary a matsayin mai ƙididdigewa don yin la'akari da danniya na hanta da kuma ƙwayar ƙwayoyi.An gwada mahadi guda huɗu waɗanda aka san suna canza matakan GSH da GSSG a cikin wannan binciken.Diquat (diquat dibromide monohydrate) da acetaminophen an gudanar da su ga berayen.Paraquat da tert-butyl hydroperoxide an gudanar da su ga mice don haifar da canje-canje na GSH da GSSG.GSH biliary da GSSG an ƙididdige su ta amfani da matakan daidaitawa da aka shirya tare da bile na wucin gadi don lissafin duk wani tasirin bile matrix a cikin binciken LC-MS kuma don guje wa tsangwama na GSH da GSSG na ƙarshe.Tare da misalai guda huɗu (a cikin berayen da beraye) na canje-canjen da aka haifar da miyagun ƙwayoyi a cikin motsin motsi na biliary GSSG/GSH, wannan binciken ya nuna yuwuwar haɓaka ƙimar amsawar fallasa dangane da ƙimar GSSG / GSH na biliary don tsinkayar damuwa na hanta.