Kayayyaki

Adenosine shine mai sarrafa sautin jijiyoyin jini.Wannan aikin na adenosine yana ƙarewa ta hanyar ɗaukarsa da metabolism ta ƙwayoyin endothelial microvascular (MVEC).Babban mai jigilar kaya wanda ke da hannu shine ENT1 (daidaitaccen jigilar jigilar nucleoside subtype 1).MVEC kuma yana bayyana jigilar nucleobase (ENBT1) wanda ke da hannu a cikin motsin salula na adenosine metabolites kamar hypoxanthine.Canje-canje a cikin ɗayan waɗannan tsarin sufuri zai yi tasiri ga bioactivity na adenosine da metabolism, gami da samuwar radicals na oxygen.MVEC ware daga kwarangwal tsoka na ENT1 (+/+) da ENT1 (-/-) berayen sun kasance sun fuskanci damuwa na oxidative wanda aka haifar da ischemia / reperfusion ko menadione.Ayyukan ayyuka na ENT1 da ENBT1 an tantance su ne bisa la'akari da sifili-trans influx kinetics na radiyo.An sami raguwa a cikin ƙimar karɓar hypoxanthine mai tsaka-tsakin ENBT1 ta hanyar ENT1 (+/+) MVEC da aka yi amfani da shi tare da menadione ko bayan bayyanar da yanayin da ke kwatanta isc hemia/reperfusion.A cikin lokuta biyu, superoxide dismutase mimetic MnTMPyP ya rage asarar ayyukan ENBT1, yana haifar da radicals superoxide a cikin martani.Ya bambanta, MVEC keɓe daga ENT1 (-/-) mice bai nuna raguwa a cikin ayyukan ENBT1 ba akan jiyya tare da menadione ko simulated ischemia / reperfusion, amma sun sami matsayi mafi girma na ayyukan catalase dangane da ENT1 (+/+) MVEC.Wadannan bayanan sun nuna cewa aikin ENBT1 ya ragu a cikin MVEC don mayar da martani ga karuwar radical superoxide wanda ke hade da raunin ischemia / reperfusion.MVEC keɓe daga ENT1 (-/-) mice ba sa nuna wannan raguwa a cikin ENBT1, maiyuwa saboda karuwar ayyukan catalase.