Kayayyaki

Tet 5-methylcytosine dioxygenases yana haifar da demethylation na DNA ta hanyar samar da 5-hydroxymethylcytosine da ƙarin samfuran oxidized.Tet1 da Tet2 an bayyana su sosai a cikin sel masu ƙarfi na linzamin kwamfuta kuma an rage su zuwa wurare daban-daban a cikin ƙwayoyin somatic, amma hanyoyin rubutun ba su da tabbas.Anan mun ayyana yankin masu talla da haɓakawa a cikin Tet1 da Tet2.A cikin 15-kb "superenhancer" na Tet1, akwai rukunin farawa guda biyu (TSSs) tare da tsarin kunnawa daban-daban yayin haɓakawa.Yankin mai talla na 6-kb a sama na TSS mai nisa yana aiki sosai a cikin sel pluripotent naive, yana ba da rahoto kai tsaye ta Tet1 a cikin tsarin transgenic, kuma cikin hanzari yana jurewa DNA methylation da yin shiru akan bambance bambancen sel na al'ada da epiblast na asali.TSS na biyu na ƙasa, wanda ke da alaƙa da mai haɓaka arziƙin CpG mai rauni, ana kunna shi ta maƙwabcin haɓakawa a cikin ƙwayoyin ƙwanƙwasa na embryonic (ESCs) da sel masu kama da epiblast (EpiLCs).Tet2 yana da romoter na tsibirin CpG tare da ayyuka masu zaman kansu da yawa da ESC takamaiman mai haɓaka intragenic na nesa;na karshen yana raguwa da sauri a cikin EpiLCs.Bincikenmu yana bayyana nau'ikan ƙa'idodin ƙa'idar rubutu a Tet1 da Tet2 yayin canjin yanayin yanayin tantanin halitta na farkon haɓakawa.Sabbin masu ba da rahoto na transgenic masu amfani da Tet1 da Tet2 cis-regulatory domains na iya yin aiki don bambance sauye-sauyen sauye-sauye a cikin jihohi masu yawa da kuma bambance-bambancen epigenetic.