Kayayyaki

An yi amfani da spectroscopy na infrared don taswirar hulɗar ma'auni-gina jiki: canje-canje na daidaituwa na sarcoplasmic reticulum Ca (2+) -ATPase akan haɗin nucleotide da ATPase phosphorylation an saka idanu ta hanyar amfani da substrate ATP da ATP analogues (2'-deoxy-ATP, 3). '-deoxy-ATP, da inosine 5'-triphosphate), waɗanda aka gyaggyarawa a takamaiman ƙungiyoyin aiki na substrate.Canje-canje ga 2'-OH, da 3'-OH, da kuma rukunin amino na adenine sun rage girman canjin haɓakar haɓakar haɓakar ATPase, tare da tasiri mai ƙarfi da aka lura ga na ƙarshe biyu.Wannan yana nuna ƙwarewar tsarin tsarin hadaddun nucleotide-ATPase zuwa hulɗar mutum ɗaya tsakanin nucleotide da ATPase.Duk ƙungiyoyin da aka yi nazari suna da mahimmanci don ɗaurewa da hulɗar ƙungiyar haɗin gwiwar da aka ba su tare da ATPase sun dogara da hulɗar sauran ƙungiyoyin ligand.An lura da phosphorylation na ATPase don ITP da 2'-deoxy-ATP, amma ba don 3'-deoxy-ATP ba.Babu wata hanyar haɗin kai tsaye tsakanin t girman canjin canji a kan ɗaurin nucleotide da ƙimar phosphorylation da ke nuna cewa cikakkiyar canjin yanayin da ATP ya haifar ba dole ba ne don phosphorylation.Kamar yadda aka lura da hadaddun nucleotide-ATPase, daidaitawar farkon phosphorylated ATPase matsakaici E1PCa (2) shima ya dogara da nucleotide, yana nuna cewa jihohin ATPase suna da ƙarancin daidaituwar daidaituwa fiye da yadda ake tsammani a baya.