Kayayyaki

The inji na Mycobacterium smegmatis G (MbsG), a flavin-dogara l-lysine monooxygenase, an bincika a karkashin tsayayye da kuma m yanayi dauki ta amfani da firamare da sauran ƙarfi kinetic isotope effects, substrate analogs, pH da sauran ƙarfi danko effects a matsayin injin bincike.Sakamakon ya nuna cewa l-lysine yana ɗaure a gaban NAD (P) H, wanda ke haifar da raguwa a cikin adadin yawan adadin flavin.l-lysine dauri ba shi da wani tasiri a kan adadin flavin oxidation, wanda ke faruwa a cikin tsari guda ɗaya ba tare da lura da tsaka-tsakin C4a-hydroperoxyflavin ba.An ƙaddara irin wannan tasirin tare da analogs na substrate da yawa.Flavin oxidation shine pH mai zaman kanta yayin da kcat / km da kred / KD pH bayanan martaba na NAD (P) H suna nuna ƙimar pKa ɗaya na ~ 6.0, tare da haɓaka aiki yayin da pH ke raguwa.A ƙasan pH, enzyme ɗin ya zama wanda ba a haɗa shi ba, yana samar da ƙarin hydrogen peroxide da superoxide.Canja wurin Hydride yana ɗan iyakance ƙimar ƙima a pH mai tsaka tsaki kuma ya zama mafi ƙayyadaddun ƙimar a lo w pH.An lura da tasirin danko mai juzu'i akan kcat/Km don NAD(P)H a tsaka tsaki pH yayin da aka ga tasirin danko na yau da kullun a ƙananan pH.Tare, sakamakon yana nuna wata hanya ta musamman inda ƙayyadaddun ƙayyadaddun ƙayyadaddun ƙayyadaddun ƙayyadaddun ƙayyadaddun ƙididdiga da pH-m canji ya faru a cikin raƙuman raƙuman raƙuman ruwa, wanda ke rinjayar ingancin lysine hydroxylation.