Kayayyaki

Babban abin da ke haifar da kamuwa da cutar Clostridium difficile (CDI) shine amfani da maganin rigakafi saboda rushewar ma'auni na microbiota mai masaukin baki.Don adana ƙwayoyin probiotic masu amfani masu amfani a lokacin maganin kamuwa da cuta, amfani da kwayoyin halitta tare da zaɓin aikin ƙwayoyin cuta yana haɓaka tasiri ta hanyar zaɓin cire C. difficile.Ɗaya daga cikin su shine alkaloid 8-hydroxyquinoline (8HQ), wanda aka nuna don hana clostridia da zabi ba tare da danne bifidobacteria ba.Ana gwada zaɓin aikin rigakafin ƙwayoyin cuta gabaɗaya ta dabarun al'ada na nau'ikan ƙwayoyin cuta guda ɗaya.Koyaya, babban ƙayyadaddun waɗannan fasahohin shine rashin iya bayyana bambancin haɓakar haɓakar haɓakar nau'ikan ƙwayoyin cuta a cikin al'adu ɗaya a cikin gwaji iri ɗaya.A cikin binciken da muke ciki, mun haɗu da kyalli a cikin yanayin yanayin yanayi da cytometry mai gudana don bayyana canje-canje a cikin sel masu aiki da marasa aiki na gaurayawan al'ada da aka kafa ta hanyar samun dama ta pathogen C. diff icile CECT 531 da Bifidobacterium longum subsp mai fa'ida.longum CCMDMND BL1 bayan fallasa zuwa 8HQ.An lura cewa ba tare da 8HQ ba, rabon nau'ikan nau'ikan biyu ya kusan daidai, yana motsawa tsakanin 22.7 da 77.9 % a lokacin ɓacin lokaci na 12 h, yayin da tare da 8HQ rabon C. difficile mai aiki ya ragu bayan 4 h, kuma ya ci gaba tsakanin 8.8 kawai. kuma 17.5%.Sabanin haka, ci gaban bifidobacterial bai damu da 8HQ ba.Sakamakon wannan binciken ya nuna tasirin hanawa na zaɓi na 8HQ akan haɓakar haɓakar haɓakar haɓakar clostridial da bifidobacterial, da yuwuwar wannan fili don haɓaka wakilai masu zaɓi don sarrafa CDI.