Kayayyaki

Serine / threonine sunadaran phosphatases an bayyana su a cikin ƙwayoyin cuta masu yawa a matsayin mahimman enzymes da ke cikin hanyoyin watsa siginar da ke dogara da phosphorylation kuma akai-akai hade da virulence na waɗannan kwayoyin halitta.Binciken Mycoplasma synoviae genome ya nuna kasancewar wata kwayar halitta (prpC) da ke ɓoye wani furotin phosphatase na furotin phosphatase 2C (PP2C) subfamily.Anan, mun bayar da rahoton cikakken sifa na sinadarai na M. synoviae phosphatase (PrpC) da kuma muhimmiyar rawa na ions ƙarfe a cikin tsarin tsarin-aiki na wannan enzyme.Binciken jerin amino acid na PrpC ya nuna cewa duk ragowar da ke cikin cibiyar ƙarfe ta dinuclear da ragowar ƙarfe na haɗin gwiwar ƙarfe na uku, waɗanda aka adana a cikin PP2C phosphatases, suna nan a cikin PrpC.PrpC furotin ne na monomeric wanda zai iya lalata abubuwan phosphorylate tare da dogaro da Mn(2+) ions.Binciken kwanciyar hankali na thermal ya nuna kwanciyar hankali na enzyme a yanayin zafi mai sauƙi da ƙarancin ions na Mn(2+) a cikin wannan kadarar.Mass spectrometry bincike ya nuna cewa ions ƙarfe uku suna ɗaure zuwa PrpC, biyu daga cikinsu tare da tabbataccen tsayin daka.Binciken mutagal na ragowar haɗin ƙarfe na uku, Asp122 da Arg164, ya bayyana cewa waɗannan bambance-bambancen sun nuna raunin dauri na ions manganese, kuma duka maye gurbi sun shafi ayyukan PrpC phosphatase.Dangane da waɗannan sakamakon, PrpC memba ne na furotin phosphatase mai dogaro da ƙarfe tare da ingantacciyar kwanciyar hankali a cikin sigar holo kuma tare da Asp122, mai yuwuwa an haɗa shi a cikin rukunin daurin ƙarfe na uku, mai mahimmanci ga ayyukan catalytic.