Kayayyaki

Binciken na yanzu yana bayyana hanya mai sauƙi da inganci ta amfani da babban aikin chromatography na ruwa (HPLC) haɗe zuwa gano haske don tantance sigogin motsin motsi na glutamate a cikin nama mai juyayi.Nama na retina da aka samu daga kajin 7-day-day an haɗa su tare da sanannun ƙididdiga na glutamate (50-2000 μM) don 10 min, kuma an auna matakan o-phtaldehyde (OPA) -wanda aka samu neurotransmitter a cikin matsakaicin shiryawa.Ta hanyar yin la'akari da bambanci tsakanin ƙaddamarwa na farko da na ƙarshe na glutamate a cikin matsakaici, an kwatanta tsarin da za a iya ɗauka (K (m) = 8.2 da V (max) = 9.8 nmol / mg protein / min).Wannan ma'auni ya kasance galibin sodium- da dogaro da zafin jiki, yana ba da ƙarfi da ƙarfi cewa tsarin raguwar maida hankali da gaske ke ɗauka ta manyan masu jigilar kaya.Ƙara zuwa wannan, sakamakonmu ya kuma nuna cewa zinc chloride (mai hana glutamate/aspartate transporters) ya haifar da raguwa mai dogara da hankali a cikin glutama te, yana nuna ƙayyadaddun tsarin mu.Gabaɗaya, aikin na yanzu yana nuna wata hanya ta dabam don kimanta ɗaukar glutamate a cikin nama mai juyayi ta amfani da HPLC.Wannan tsarin zai iya zama kayan aiki mai mahimmanci don nazarin da ke hade da halayyar sauye-sauye na minti daya a cikin jigilar glutamate da ke da alaka da raunin tsarin juyayi na tsakiya.