Kayayyaki

1.Early T-cell precursor leukemia (ETP-ALL) wani nau'in cutar sankarar bargo ne mai hatsarin gaske wanda ba a fahimta sosai a matakin kwayoyin.Anan mun ba da rahoton jujjuyawar da aka yi niyya ga ma'aunin rubutu na ZEB2 mai yatsar yatsa E-box a matsayin cutar da ke faruwa a cikin rashin girma/ETP-ALL.Yin amfani da samfurin linzamin kwamfuta na sharadi-na aiki, muna nuna cewa dorewar magana ta Zeb2 tana fara cutar sankarar mahaifa ta T-cell.Haka kuma, cutar sankarar linzamin kwamfuta da Zeb2 ke tukawa tana nuna wasu fasalulluka na sa hannun bayyanar ɗan adam da bai balaga ba/ETP-ALL, da kuma ingantaccen haɓakar cutar sankarar bargo da kunna Janus kinase (JAK) / masu fassarar sigina da masu kunna aikin rubutu (STAT) suna sigina ta hanyar. Kunna kwafi na IL7R.Wannan binciken ya bayyana ZEB2 a matsayin oncogene a cikin ilmin halitta na rashin balaga / ETP-ALL kuma yana ba da hanya zuwa ga binciken farko na asibiti na mahaɗan labari don maganin wannan mummunan nau'in T-ALL na ɗan adam ta amfani da samfurin linzamin kwamfuta na Zeb2.

2.Pluripotency, sabuntawar kai, da bambance-bambance sune siffofi na musamman na ƙwayoyin embryonic (ES), don haka suna ba da ra'ayi mai mahimmanci a cikin maganin farfadowa.Hanyoyin haɓakawa suna buƙatar ƙungiyar da aka daidaita, galibi ana tsara su ta hanyar sanannun JAK/STAT, PI3K/AKT, da hanyoyin ERK/MAPK.An gano SPREDs (sunan sunadaran da ke da alaƙa tare da yankin EVH1) a matsayin masu hanawa na hanyar siginar ERK / MAPK, yayin da babu wani abu da aka sani game da ayyukan su a cikin sel ES da kuma lokacin farkon bambance-bambancen, ya zuwa yanzu.Mun haifar da SPRED1 da SPRED2 overexpressing da SPRED2 knockout murine ES Kwayoyin don nazarin ayyukan sunadaran SPRED a cikin sel ES da kuma lokacin bambance-bambancen farko.Ƙarfafawa na SPREDs yana ƙaruwa sosai da sabuntawar kai da kuma clonogenicity na murine ES Kwayoyin, yayin da rashin SPRED2 yana rage yaduwa kuma yana ƙara apoptosis.A lokacin bambance-bambancen farko a cikin jikin amfrayo, SPREDs suna haɓaka yanayi mai ƙarfi kuma suna hana bambancewa ta yadda mesodermal d ifferentiation cikin cardiomyocytes ke jinkiri sosai kuma an hana shi.LIF- da haɓaka haɓaka haɓaka ya nuna cewa SPEDs sun hana kunna ERK / MAPK a cikin ƙwayoyin ES na murine.Duk da haka, babu wani tasiri da aka gano akan kunnawar LIF na JAK / STAT3, ko PI3K / AKT hanyar siginar siginar ta hanyar sunadaran SPRED.Muna nuna cewa SPREDs suna inganta sabuntawar kai da kuma hana bambancin mesodermal na murine ES Kwayoyin ta hanyar zaɓin zaɓi na ERK / Hanyar siginar MAPK a cikin sel masu ƙarfi.