Kayayyaki

PMSF shine mai hanawar serine/cysteine ​​​​protease mai hanawa wanda aka saba amfani dashi a cikin shirye-shiryen sel lysates.

Nazarin in vitro: PMSF (2 mM) ya hana tarawar inositol phosphate mai motsa carbachol da kawai 15% -19% a gaban Li +.Hana juyar da phosphoinotide ta PMSF shine saboda matakai ɗaya ko fiye da ke biyo bayan rushewar phosphoinotides [1].PMSF yana hana acylation na ragowar inositol na tsaka-tsakin GPI a cikin jini na T. brucei.PMSF yana hana samuwar glycolipid C amma ba sake fasalin fatty acid a cikin vitro ba.PMSF yana hana ƙari na GPI acylation da ethanolamine phosphatase a cikin procyclic trypanosomes, amma ba a cikin ƙwayoyin Hela [2].

A cikin nazarin vivo: PMSF (0.1 mL / 10 g b.wt, ip) ya haifar da antinociception, kamar yadda aka nuna ta hanyar haɓaka mai amsawa a cikin% MPE a cikin ƙima na rashin jinkirin wutsiya, amma ya kasa samar da ƙayyadaddun ƙayyadaddun ƙwayar mota mai amsawa.Mice waɗanda suka karɓi allurar intraperitoneal na PMSF sun nuna tasirin cannabinoid ciki har da antinociception, hypothermia da rashin motsi tare da ƙimar ED50 na 86, 224 da 206 mg/kg, bi da bi.PMSF (30 mg / kg) pretreatment yana haɓaka tasirin anandamide akan amsawar wutsiya (antinociception), ayyukan locomotor da motsi ta 5-ninka, 10-ninka da 8-ninka, bi da bi [3].

Gwajin dabba: An yi amfani da berayen ICR maza masu nauyin 18 zuwa 25 g a cikin tantancewar.PMSF an narkar da shi a cikin man sesame kuma ana gudanar da shi a cikin ɓangarorin 0.1 mL/10 g b.wt.Koyaushe gudanar da PMSF mintuna 10 kafin anandamide na cikin jijiya ko allurar abin hawa.An kai ɓeraye zuwa ɗakin tantancewar dare ɗaya ba tare da an sami katsewar abinci ko ruwa ba.Bayan anandamide na ciki ko sarrafa abin hawa, an kimanta kowace dabba kamar haka: 5 mintuna don jinkirin wutsiya (antinociceptive) amsa da 5 zuwa 15 mintuna don ayyukan gaggawa (motar);ko minti 5 don zafin jiki na tsakiya (duba) da zobe Immobilization (catalepsy) na mintuna 5 zuwa 10.