Kayayyaki

Nazarin chromatin immunoprecipitation (ChiP) mai fa'ida ga kwayoyin halitta ya kawo haske mai mahimmanci game da yanayin halittar kwayoyin halittar sunadaran da ke da alaƙa da chromatin da gyare-gyaren tarihi.Yawan adadin bayanai da aka samar ta hanyar waɗannan nazarin, duk da haka, yana buƙatar hanyoyin da ke ba da damar ingantaccen inganci da kuma nazarin mahimmancin nazarin halittu.Bugu da ƙari, har yanzu akwai furotin da maƙasudin gyare-gyare waɗanda ke da wahalar gano ta amfani da daidaitattun hanyoyin ChIP.Don magance waɗannan al'amurra, mun ƙirƙiri wani tsari na rigakafi na chromatin nan da nan wanda muke kira ZipChip.ZipChip yana rage lokaci sosai kuma yana ƙaruwa da hankali yana ba da izinin saurin dubawa na loci da yawa.Anan mun bayyana yadda ZipChIP ke ba da damar gano gyare-gyare na histone (H3K4 mono- da trimethylation) da yisti histone demethylases guda biyu, Jhd2 da Rph1, waɗanda a baya da wuya a gano ta amfani da daidaitattun hanyoyin.Bugu da ƙari kuma, muna nuna versatility na ZipChIP ta hanyar nazarin wadatar histone deacet ylase Sir2 a heterochromatin a cikin yisti da wadatar da chromatin remodeler, PICKLE, a euchromatin a Arabidopsis thaliana.