Kayayyaki

Streptomyces ambofaciens yana hada spiramycin na macrolide.Tarin kwayoyin halitta na biosynthetic don spiramycin an kwatanta shi don S. ambofaciens.Baya ga tsarin halittar srmR (srm22), wanda aka gano a baya (M. Geistlich et al., Mol. Microbiol. 6: 2019-2029, 1992), an gano ƙwayoyin ƙwayoyin cuta masu sakawa guda uku ta hanyar bincike na jeri.Binciken maganganun kwayoyin halitta da gwaje-gwajen rashin kunna kwayoyin halitta sun nuna cewa daya ne kawai daga cikin wadannan kwayoyin halitta guda uku, srm40, ke taka muhimmiyar rawa wajen daidaita yanayin biosynthesis na spiramycin.Rushewar srm22 ko srm40 ya kawar da samar da spiramycin yayin da wuce gona da iri ya karu da samar da spiramycin.An gudanar da nazarin magana ta hanyar juzu'i-PCR (RT-PCR) don duk kwayoyin halittar tari a cikin nau'in daji da kuma a cikin srm22 (srmR) da srm40 maye gurbi.Sakamako daga nazarin magana, tare da waɗanda daga gwaje-gwajen haɓakawa, sun nuna cewa ana buƙatar Srm22 don maganganun srm40, Srm40 kasancewa takamaiman mai kunnawa ta hanya wanda ke sarrafa mafi yawan, idan ba duka ba, na spiramycin biosynthetic genes.