Kayayyaki

An gudanar da wannan binciken don bincika tasirin haɓakar kayan lambu na peptides na kayan lambu a kan sel masu girma (ASCs) a cikin rashin ƙwayar cuta da kuma hanyoyin da za su iya aiki.Yaɗuwar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar cuta (CB-MSCs) da aka yi tare da peptides na soya an gano ya karu sosai akan 3- (4,5-dimethylthiazol-2-yl). -2,5-diphenyltetrazolium bromide da Click-iT 5-ethynyl-2'-deoxyuridine kwarara cytometry kima.Bugu da ƙari, peptides na soya sun haifar da matakan phosphorylation na p44/42 MAPK (ERK), mammalian manufa na rapamycin (mTOR), p70 S6 kinase, S6 ribosomal protein (S6RP) da eukaryotic initiation factor 4E (eIF4E) a cikin ADSCs.Bugu da ƙari kuma, ƙididdigar ƙididdiga na cytokines ya nuna cewa samar da canza yanayin girma-beta1 (TGF-β1), haɓakar haɓakar haɓakar ƙwayar cuta da interleukin-6 ya karu sosai don amsawa tare da peptides na soya a cikin ADSCs da CB-MSCs.Hakazalika, phosphorylation na soya peptide na hanyar ERK / mTOR / S6RP / eIF4E an katange shi don mayar da martani ga pretreatment tare da PD98059, wani mai hana ERK.Bugu da ƙari, hana TGF-β1 ta hanyar PD98059 pretreatment da raguwa a jere a cikin yaduwar ADSC ya nuna cewa TGF-β1 yana haifar da phosphorylation na mTOR / S6RP / eIF4E.Gabaɗaya, sakamakon wannan binciken ya nuna cewa samar da TGF-β1 da ke dogara da ERK yana taka muhimmiyar rawa a cikin haɓakar ADSC da ke haifar da peptide a ƙarƙashin sharuɗɗan marasa amfani. Haƙƙin mallaka © 2012 Elsevier Inc. Duk haƙƙin mallaka.