Bicyclo isomeric guda biyu [4.1.0] heptane analogues na glycosidase inhibitor galacto-validamine, (1R *,2S,3S,4S,5S,6S*) -5-amino-1- (hydroxymethyl) bicyclo[4.1.0] heptane -2,3,4-triol, an haɗa su a cikin matakai 13 daga 2,3,4,6-tetra-O-benzyl-D-galactose.Ayyukan hanawa na amines guda biyu da aka ƙayyade, da kuma acetamides masu dacewa, an auna su akan kasuwancin alpha-galactosidase enzymes daga kofi na kofi da E. coli.Ayyukan glycosyl hydrolase iyali GH27 enzyme (kofi wake) an hana shi gasa ta hanyar 1R,6S-amine (7), hulɗar ɗaure wanda ke da darajar K (i) na 0.541 microM.GH36 E. coli alpha-galactosidase ya nuna ma'amala mai rauni da yawa tare da 1R,6S-amine (IC(50)= 80 microM).Diastereomeric 1S, 6R-amine (9) daure da rauni ga duka galactosidases, (kofi wake, IC (50) = 286 microM) da (E. coli, IC (50) = 2.46 mM).
