Wani labari β-glucosidase gene, bgl1G5, an cloned daga Phalophora sp.G5 kuma an yi nasarar bayyana shi a Pichia pastoris.Binciken jeri ya nuna cewa kwayar halitta ta ƙunshi buɗaɗɗen firam ɗin karatu 1,431-bp mai ɓoye furotin na amino acid 476.Jerin amino acid da aka cire na bgl1G5 ya nuna babban asalin 85% tare da halayen β-glucosidase daga Humicola grisea na dangin glycoside hydrolase 1. Idan aka kwatanta da sauran takwarorinsu na fungal, Bgl1G5 ya nuna irin wannan aiki mafi kyau a pH 6.0 da 50 ° C kuma ya kasance barga. pH 5.0-9.0.Bugu da ƙari, Bgl1G5 ya nuna kyakkyawan yanayin zafi a 50 ° C (rabin rayuwa 6) da takamaiman aiki (54.9 U mg-1).Ma'auni na K m da V zuwa p-nitrophenyl β-D-glucopyranoside (pNPG) sun kasance 0.33 mM da 103.1 μmol min-1 mg-1, bi da bi.Ƙididdigar ƙayyadaddun ƙayyadaddun ƙayyadaddun ƙayyadaddun ƙayyadaddun abu ya nuna cewa Bgl1G5 yana da ƙarfi sosai a kan pNPG, mai rauni akan p-nitrophenyl β-D-cellobioside (pNPC) da p-nitrophenyl-β-D-galactopyranoside (ONPG), kuma ba shi da wani aiki akan cellobiose.
