Halin beta-glucosidase (bgl3) daga Streptomyces sp.QM-B814 (Tarin Al'adu na Nau'in Amurka 11238) an rufe shi ta hanyar haɓaka aikin beta-glucosidase-mutant mutant na Streptomyces lividans.An sami buɗaɗɗen firam ɗin karatu na nucleotides 1440 da ke ɓoye polypeptide na amino acid 479 ta hanyar jeri.Sunan furotin da aka ɓoye (Bgl3) yana nuna kamanceceniya mai yawa (fiye da 45% ainihi) tare da beta-glycosidases daga iyali-1 glycosyl hydrolases.Enzyme cloned, wanda aka tsarkake bayan hazo ammonium sulphate da matakai guda biyu na chromatographic, monomeric ne tare da adadin kwayoyin halitta 52.6 kDa, kamar yadda aka ƙaddara ta hanyar ƙididdigar taro, da madaidaicin isoelectric na pI 4.4.Enzyme ya bayyana a matsayin beta-glucosidase tare da ƙayyadaddun ƙayyadaddun kayan aiki, yana aiki akan celloligomers, kuma yana aiwatar da halayen transglycosylation.Ƙimar Km da aka kiyasta ga p-nitrophenyl-beta-D-glucopyranoside da cellobiose sune 0.27 mM da 7.9 mM, bi da bi.Kimar Ki don glucose da delta-gluconolactone, ta yin amfani da p-nitrophenyl-beta-D-glucopyranoside a matsayin substrate, sune 65 mM da 0.08 mM, bi da bi.Enzyme da aka tsarkake yana da mafi kyawun pH na pH 6.5 kuma mafi kyawun zafin jiki don aiki shine digiri 50.
