Bacteroides thetaiotaomicron ya misalta, suna taka muhimmiyar rawa wajen kiyaye lafiyar ɗan adam ta hanyar amfani da manyan iyalai na glycoside hydrolases (GHs) don cin gajiyar polysaccharides na abinci da masu karɓar glycans azaman abubuwan gina jiki.Irin wannan fadada iyali na GH ana misalta shi ta dangin 23 GH92 glycosidases wanda B. thetaiotaomicron genome ya tsara.Anan mun nuna cewa waɗannan alpha-mannosidases ne waɗanda ke aiki ta hanyar ƙaura guda ɗaya don amfani da N-glycans mai masaukin baki.Tsarin nau'i-nau'i uku na mannosidases GH92 guda biyu yana bayyana dangin sunadaran sunadaran yanki guda biyu wanda cibiyar catalytic ke samuwa a cikin yanki na yanki, samar da acid (glutamate) da tushe (aspartate) taimako ga hydrolysis a cikin Ca (2+) - hanyar dogara.Tsarin sassa uku na GH92s a cikin hadaddun tare da masu hana hanawa suna ba da haske game da ƙayyadaddun ƙayyadaddun tsari, tsari da tsarin hanyar daidaitawa na catalysis.Ca (2+) yana taka muhimmiyar rawa wajen taimakawa karkatar da mannoside daga ƙasa-jihar (4)C (1) kujera daidaitawa zuwa yanayin canji.
