Kayayyaki

Protein phosphorylation yana sarrafa ayyuka daban-daban na salon salula kuma yana taka muhimmiyar rawa a farkon ci gaban tsirrai.Don haɓakawa da faɗaɗa binciken da aka yi a baya game da furotin phosphorylation a cikin tsiron Arabidopsis mun yi amfani da wata hanya dabam wacce ta haɗu da haɓakar furotin a ƙarƙashin yanayin rashin ƙarfi tare da haɓakar ƙarfe-ion affinity chromatography (IMAC) na wadatar phosphoproteins mara kyau a cikin rubisco-depleted tsantsa, sannan ganowa. ta amfani da gel electrophoresis mai girma biyu (2-DE) da ruwa chromatography-tandem mass spectrometry (LC-MS/MS).In-gel trypsin narkewa da bincike na zaɓaɓɓen gel spots gano 144 phosphorylated peptides da ragowar, wanda kawai 18 phosphopeptides da 8 phosphosites aka samu a cikin PhosPhAt 4.0 da P3DB Arabidopsis thaliana phosphorylation shafukan bayanai.Fiye da rabin 82 da aka gano phosphoproteins sun shiga cikin metabolism na carbohydrate, photosynthesis/numfashi ko mechani sms na amsa damuwa.Ingantattun phosphoproteins masu inganci kafin 2-DE da LC-MS/MS ya bayyana yana haɓaka gano phosphorylated threonine da ragowar tyrosine idan aka kwatanta da hanyoyin da ke amfani da haɓaka matakin peptide, yana nuna cewa hanyoyin biyu sun ɗan dace dangane da ɗaukar hoto.Kwatanta sakamakon da aka samu ga matasa masu tsiro da waɗanda aka samu a baya don balagagge ganyen Arabidopsis sun gano sunadaran sunadarai guda biyar waɗanda aka bambanta da phosphorylated a cikin waɗannan kyallen takarda, wanda ke nuna yuwuwar wannan dabarar don bincikar kuzarin furotin phosphorylation yayin haɓaka shuka.