Biosynthesis na proteoglycans da glycosaminoglycans a gaban p-nitrophenyl-xyloside an yi nazari ta hanyar amfani da tsarin al'adun kwayar halitta na granulosa na bera na farko.Ƙara p-nitrophenyl-xyloside a cikin matsakaiciyar al'ada ta salula ya haifar da kusan 700% karuwa na [35S] sulfate incorporation (ED50 a 0.03 mM) a cikin macromolecules, wanda ya haɗa da sarƙoƙi na chondroitin sulfate kyauta wanda aka fara akan xyloside da 'yan asalin proteoglycans.Sarƙoƙin sulfate na chondroitin kyauta waɗanda aka fara akan xyloside kusan an ɓoye su a cikin matsakaici.Girman kwayoyin halittar sarkar sulfate na chondroitin sulfate ya ragu daga 40,000 zuwa 21,000 yayin da aka haɓaka jimlar [35S] sulfate haɗin gwiwa, yana nuna cewa haɓakar haɓakar sulfate na chondroitin ya rikitar da tsarin al'ada na ƙarshen sarkar glycosaminoglycan.Biosynthesis na heparan sulfate proteoglycans an rage da kusan 50%, mai yiwuwa saboda gasa a matakin UDP-sugar precursors.[35S] An rufe haɗin sulfate ta hanyar ƙara cycloheximide tare da farkon rabin lokacin kusan 2 hr a gaban xyloside, yayin da in babu xyloside ya kasance kusan 20 min.Bambanci mai yiwuwa yana nuna yawan jujjuyawar iyawar glycosaminoglycan gabaɗaya.Adadin jujjuyawar ƙarfin haɓakar glycosaminoglycan da aka gani a cikin ƙwayoyin granulosa na ovarian ya fi guntu fiye da abin da aka lura a cikin chondrocytes, yana nuna ikon dangi na ayyukan biosynthetic na proteoglycan a cikin jimlar ayyukan rayuwa na sel.
