Kwayar cutar entomopathogenic, Xenorhabdus nematophila, yana haifar da rigakafin rigakafi na kwari da aka yi niyya ta hanyar hana ayyukan phospholipase A(2) (PLA(2)).Kwanan nan, an gano kwayar cutar PLA(2) mai alaƙa da rigakafi daga ƙwayar ƙwaro na ja, Tribolium castaneum.Wannan binciken ya rufe wannan nau'in PLA(2) a cikin wani nau'in magana na kwayan cuta don samar da enzyme recombinant.The recombinant T. castaneum PLA (2) (TcPLA (2)) ya nuna halinsa na enzyme aiki tare da substrate maida hankali, pH, da na yanayi zazzabi.Siffofin sinadarai na sinadarai sun dace da nau'in sirrin PLA (2) (sPLA (2)) saboda an hana aikin sa ta dithiothreitol (wani wakili mai rage disulfide bond) da bromophenacyl bromide (mai hana sPLA (2) na musamman) amma ba ta methylarachidonyl ba. fluorophosphonate (wani takamaiman nau'in cytosolic na PLA (2)).Broth na al'ada na X. nematophila ya ƙunshi PLA (2) abubuwan hanawa (s), wanda ya fi yawa a cikin kafofin watsa labaru da aka samu a wani lokaci mai girma na ƙwayoyin cuta.Matsalolin hanawa na PLA(2) ya kasance mai juriya da zafi kuma an fitar dashi a cikin ɓangarorin ruwa da na halitta.Tasirin wani yanki mai hanawa na PLA (2) akan rigakafin rigakafi na T. castaneum daidai yake da wanda ya haifar da hana bayyanar TcPLA (2) ta hanyar tsoma baki RNA.
