Kayayyaki

Ana bincika polycations azaman masu ɗaukar hoto don sadar da acid nucleic na warkewa.Polycations ne na al'ada inert pharmacological tare da kawai aikin isar da kayan warkewa.Wannan binciken ya ba da rahoton haɗin kai na polycation mai hana kansa (DSS-BEN) bisa ga maganin polyamine analogue N(1), N (11) -bisethylnorspermine (BENSpm).An ƙera polycation ɗin don yin aiki bibiyu azaman mai jigilar kwayoyin halitta da kuma ƙwararrun ƙwayoyi masu niyya dysregulated polyamine metabolism a cikin ciwon daji.Yin amfani da haɗin NMR da HPLC, mun tabbatar da cewa polycation mai kashe kansa yana fuskantar lalacewa ta cikin salula a cikin magungunan mahaifa na BENSpm.BENSpm da aka saki yana rage matakan salula na spermidine da maniyyi kuma yana haɓaka polyamine catabolic enzymes spermine / spermidine N (1) -acetyltransferase (SSAT) da kuma spermine oxidase (SMO).Haɗaɗɗen polycations suna samar da polyplexes tare da DNA kuma suna sauƙaƙe haɓaka mai inganci.Yin amfani da damar BENSpm don wayar da kan kwayoyin cutar kansa zuwa apoptos da ke haifar da TNFa shine, muna nuna cewa DSS-BEN yana haɓaka aikin kashe kwayoyin halittar TNFa.Sakamakon da aka ruwaito ya tabbatar da DSS-BEN a matsayin tsarin isar da ayyuka guda biyu wanda zai iya sadar da kwayar halitta ta warkewa da kuma inganta sakamakon maganin kwayoyin halitta a sakamakon lalacewar intracellular DSS-BEN zuwa BENSpm da kuma sakamako mai amfani na BENSpm akan polyamine dysregulated. metabolism a cikin ciwon daji.