Kayayyaki

C6 dicarboxylic acid, adipic acid, shine muhimmin sinadari mai mahimmanci a masana'antu.Samar da sinadarin adipic acid shine kyakkyawan zaɓi ga hanyar petrochemical na yanzu.Anan, mun bayar da rahoton biosynthesis na adipic acid ta amfani da hanyar wucin gadi da aka yi wahayi ta hanyar juyawar beta-oxidation na acid dicarboxylic.Hanya ta biosynthetic ta ƙunshi haɗaɗɗun acetyl-CoA da succinyl-CoA don samar da kashin baya na C6 da raguwa na gaba, bushewa, hydrogenation, da sakin adipic acid daga thioester.An fara gwada hanyar a cikin vitro tare da gyare-gyaren hanyoyin enzymes sannan kuma an gabatar da aikin a cikin Escherichia coli don biosynthesis da fitar da adipic acid a cikin al'ada.An haɓaka titer ɗin samarwa da kusan ninki 20 ta hanyar haɗakar da enzymes waɗanda suka fi dacewa don haɓaka halayen haɗin gwiwa da ƙara samun isar da iskar gas.Wannan aikin yana nuna biosynthesis kai tsaye na adipic acid ta hanyar da ba na halitta ba, wanda zai iya ba da damar samar da sabuntawa.