Kayayyaki

Metabolite na kwayan cuta na hanji na ginseng protopanaxadiol saponin, 20-O- (β-D-glucopyranosyl) -20 (S) -protopanaxadiol (compound K), an bayar da rahoton haifar da apoptosis a cikin nau'ikan ƙwayoyin cutar kansa.Koyaya, ainihin hanyoyin da mahadi K ke haifar da su a cikin ƙwayoyin cutar hanta na hanta (HCC) ba su da tabbas.Domin bincika yiwuwar hanyoyin apoptotic, mun bincika tasirin anticancer na fili K a cikin MHCC97-H.Binciken MTT ya nuna cewa fili K ya hana haɓakar ƙwayoyin MHCC97-H tare da ƙananan ƙwayar cuta a cikin ƙwayoyin hepatoma na al'ada.Ci gaban zagayowar tantanin halitta da tabon tantanin halitta sun nuna karuwa a cikin juzu'in apoptotic sub-G1.Jiyya na MHCC97-H tare da fili K kuma ya haifar da raguwa a cikin yuwuwar mitochondrial membrane (Δψm) da lalata DNA.Ƙarin binciken ya nuna cewa fili K ya haɓaka Fas, FasL, Bax / Bcl-2 rabo da pro-caspase-9, pro-caspase-3 a cikin hanyar dogara da kashi, kuma ya hana Akt phosphorylation.Wadannan sakamakon sun nuna cewa fili na K yana hana yaduwar kwayar halitta kuma yana haifar da apoptosis a cikin kwayoyin MHCC97-H ta hanyar Fas- da mitochondria-mediated caspase-dependent paths a cikin kwayoyin HCC na mutum.