Kayayyaki

An bayyana hanyar tabo don amfani tare da sassan nama na glycol methacrylate wanda ba ya lalata abin da ke cikin filastik ko cire sassan daga nunin faifan gilashi.Rini na asali shine celestine blue B. An shirya shi ta hanyar zalunta 1 g na rini tare da 0.5 ml maida hankali sulfuric acid.Sannan a narkar da shi tare da mafita mai zuwa.Ƙara 14 ml glycerin zuwa 100 ml 2.5% ferric ammonium sulfate kuma dumi bayani zuwa 50 C. A ƙarshe daidaita pH zuwa 0.8 zuwa 0.9 Maganin lalata acid ya ƙunshi 0.075% ponceau de xylidine da 0.025% acid fuchsin a cikin 10% acetic acid.Zane-zanen da ke ɗauke da busassun robobin filastik ana nutsar da su a cikin ruwan shuɗi na celestine na tsawon mintuna biyar kuma a cikin maganin ponceau-fuchsin na tsawon mintuna goma tare da kurkura ruwa mai tsaka-tsaki.Bayan wankewar ƙarshe, sassan an bushe iska kuma an rufe su.Wannan hanyar tabo tana canza launin kyallen jikin jiki kusan iri ɗaya da hanyoyin hematoxylin da eosin.