Kayayyaki

Adsorption na sunadaran zuwa m-ruwa musaya yawanci samu empirically don inganta samuwar mai narkewa aggregates da girma, subvisible, da bayyane barbashi, amma key matakai a cikin wannan tsari sau da yawa wuya a bincika kai tsaye.Tarin da aka yi sulhu ta hanyar adsorption zuwa musaya na ruwa-silicon oxide (SiOx), daidai da saman gilashin ruwa, an siffanta shi azaman aikin pH da ƙarfin ionic don alpha-chymotrypsinogen (aCgn) da kuma don rigakafin monoclonal (IgG1).Tantanin halitta mai gudana ya ba da izinin kyamar neutron don yadudduka sunadaran da aka tallata don tsaftace saman SiOx, da kuma bayan matakan "kurkure" na gaba.Tari da aka samu a cikin bayani bayan a hankali "kurkure" saman an siffanta su da tarwatsawar neutron, microscopy, da spectroscopy na fluorescence.Kwayoyin IgG1 sun daidaita da farko "lebur" a kan saman SiOx, tare da farantin furotin na farko da aka lalata zuwa ɗan ƙaramin ƙarfi, yayin da mai yatsa mai yatsa ya kasance cikin sauƙi.Kwayoyin aCgn sun kasance masu juriya ga ɓarna lokacin da suka bayyana cewa za a buɗe su a wurin dubawa, amma an cire su cikin sauƙi.Ga lamuran da aka sami ɗauri mai ƙarfi, sunadaran da suka yi desorb shine cakuda monomer da ƙananan adadin HMW aggregates (na aCgn) ko ɓangarorin da za a iya gani (na IgG1).Canje-canje a cikin adsorption da/ko bayyanawa tare da pH sun nuna cewa hulɗar electrostatic suna da mahimmanci a kowane yanayi.