Kayayyaki

Ƙwararren jini na jini yana ba da gudummawa ga cututtuka a cikin kumburi.Hanyoyin da ake amfani da su na yanzu don in vivo kimantawa na iya aiki dangane da wuce gona da iri na Evans Blue (EB) mai ɗaure albumin suna da wahala kuma galibi basu da hankali.Mun ƙirƙiri wani sabon salo na infrared fluorescence (IRF) don auna EB-albumin extravasation don ƙididdige iyawar jijiyoyin jini a cikin ƙirar murine.An yi nazarin ƙwayar ƙwayar ƙwayar cuta ta hanyar endotoxemia don duk gabobin jiki masu ƙarfi, ƙwaƙwalwa, fata da peritoneum ta IRF da ma'aunin abin sha na gargajiya na EB a cikin tsantsa nama.Gabashin IRF ya ƙaru a layi tare da ƙara yawan adadin EB na ciki (2.5-25 mg/kg).Nama IRF ya fi kula da tarin EB idan aka kwatanta da hanyar tushen sha.Sabili da haka, bambance-bambance a cikin ɓarnawar jijiyoyin jini da tarin EB na gabobin tsakanin lipopolysaccharide da aka yi wa maganin lipopolysaccharide da saline-maganin beraye galibi suna da mahimmanci yayin da aka bincikar tushen tushen IRF amma ba ta hanyar gano tushen abin sha ba.An gano EB a cikin dukkanin gabobin 353 da aka bincika tare da IRF amma kawai a cikin 67% (239/353) na gabobin da aka bincikar ta hanyar tsarin shayarwa, yana nuna ingantaccen ƙwarewar gano EB a cikin gabobin tare da IRF.Sabanin haka, EB a cikin jini bayan gudanar da EB an auna shi da sauri ta hanyoyi biyu tare da babban alaƙa tsakanin hanyoyin biyu (n=116, r2=0.86).Ƙididdigar bambance-bambancen EB-IRF na musamman na gabobin jiki saboda endotoxin ya kasance mafi kyau lokacin da aka kwatanta IRF tsakanin berayen da suka dace da nauyi, jinsi, da shekaru, kuma tare da gyare-gyare masu dacewa don nauyin gabobin jiki da EB plasma maida hankali.Musamman ma, hanyar EB-IRF tana barin gaɓoɓin gaɓoɓin gaɓoɓin histopathology na gaba.A taƙaice, EB-IRF labari ne, mai matuƙar kulawa, sauri, kuma hanya mai dacewa don ƙayyadaddun dangi na EB a cikin gabobin jiyya tare da sarrafa beraye.