Kayayyaki

1.Metabolite profiling hanyoyin su ne muhimman kayan aiki don auna metabolite tafkunan a cikin nazarin halittu tsarin.Duk da yake mafi yawan hanyoyin siffantawa metabolite suna ba da rahoton ƙarfin dangi ko kuma dogaro da ƴan ma'auni na ciki da ke wakiltar duk metabolites, babban abin da ake buƙata don bayanin adadi mai yawa na tafkin metabolite a cikin ƙwayoyin halitta da ruwaye shine cikakken ƙudurin maida hankali.Muna ba da rahoto anan babban kayan aiki mai ƙima da iskar gas chromatography/tandem mass spectrometry (GC/MS/MS) da aka yi niyya ta hanyar siffanta metabolite wanda ke ba da cikakken ƙididdige duk abubuwan da aka gano.Hanyar ta dogara ne akan ƙaddamar da methyl chloroformate da ƙididdigewa ta hanyar spiking samfurori tare da ƙa'idodin metabolite daban wanda aka samu tare da deuterated derivatization reagents.An maye gurbin ionization na al'ada na lantarki tare da ingantaccen ionization na sinadarai tun lokacin da na ƙarshe ya fi girma ya adana ion kwayoyin halitta da sauran guntu masu nauyi na kwayoyin halitta.Wannan ya sa ya zama mai sauƙi don zaɓar keɓancewar MS/MS a tsakanin yawancin metabolites masu haɗuwa.A halin yanzu, sabuwar hanyar GC/MS/MS ta ƙunshi metabolites na farko guda 67 na gama gari waɗanda yawancinsu suna cikin ƙungiyoyin amino da nonamino Organic acid.Mun nuna yadda ake amfani da hanyar akan fitsari da samfuran jini.Hanyar ita ce babban ci gaba na hanyoyin yanzu don ƙididdigar metabolite na GC/MS na amino acid da nonamino Organic acid.

2.24S-Hydroxycholesterol (24OHC) da 27-hydroxycholesterol (27OHC) guda biyu ne masu kama da oxysterols na asali daban-daban - tsohon kusan an kafa shi ne kawai a cikin kwakwalwa kuma na ƙarshe ya kasance zuwa ƙarami a cikin kwakwalwa fiye da sauran gabobin.HUKUNCI DA AKE GWADA: Lalacewar jijiyoyi da / ko lalatawa yana haifar da haɓakar 24OHC daga kwakwalwa zuwa cikin ruwan cerebrospinal (CSF), yayin da lahani na jini-kwakwalwa yana haifar da haɓakar 27OHC daga wurare dabam dabam zuwa cikin CSF.Isotope dilution-mass. An yi amfani da spectrometry don tantance nau'in oxysterols guda biyu a cikin CSF da plasma daga fiye da 250 marasa lafiya tare da cututtuka daban-daban da cututtukan cututtuka.Marasa lafiya da ke da cututtukan demyelinating masu aiki sun ƙara matakan 24OHC a cikin CSF tare da ingantacciyar ƙimar 24OHC/27OHC.Marasa lafiya da ke fama da cutar sankarau gabaɗaya suna da manyan matakan duka steroids tare da ƙarancin 24OHC/27OHC.Marasa lafiya da ke fama da cutar Alzheimer sun ɗan ƙara yawan matakan 24OHC a cikin CSF tare da ƙarancin karuwa a 27OHC.Marasa lafiya tare da sclerosis da yawa suna da hali don samun matakan 24OHC mafi girma a lokacin lokutan aiki tare da babban rabo na 24OHC / 27OHC. Ma'auni na oxysterols guda biyu a cikin CSF da plasma na iya ƙara mahimmanci ga hanyoyin biochemical na yanzu don kimanta cututtukan cututtuka.