Kayayyaki

Makasudin wannan binciken shine siffar kwayoyin halitta da kimanta aminci na 129 Enterococcus keɓancewa da aka samu daga ruwan inabi da ke jurewa fermentation na malolactic.Halin Halitta ta DNA-PCR da aka haɓaka dazu ya nuna genotypes 23.25 keɓe wakilan duk genotypes an gano su azaman Enterococcus faecium ta takamaiman nau'in PCR da aka tantance don juriya na ƙwayoyin cuta, kasancewar ƙwayoyin ƙwayoyin cuta da ƙarfin aminobiogenic, duka a cikin matsakaici na decarboxylase da ruwan inabi.An yi nazarin ƙarfin aminobiogenic a cikin giya a gaban (kima 1) da rashi (kima 2) na Oenococcus oeni CECT 7621. Juriya ga tetracycline, cotrimoxazol, vancomycin da teicoplanin an nuna su ta hanyar 96% na nau'in, amma babu ɗayansu da ya ɗauki gwajin. kwayoyin cutar virulence.Duk nau'ikan sun ƙunshi nau'in tyrosine decarboxylase (tdc), yayin da 44% sun kasance tabbatacce ga tyramine a matsakaicin decarboxylase.Biyar daga cikin nau'ikan 25 ne kawai suka tsira a cikin ruwan inabi bayan kwana bakwai na shiryawa, kuma lokacin da HPLC ta ƙayyade abubuwan da aka samu na amines na biogenic a cikin giya, kawai waɗancan giya waɗanda nau'ikan da suka tsira sun faru sun ƙunshi amines biogenic.An gano Histamine, putrescine da cadaverine a cikin giya daga duka gwaje-gwajen, ko da yake yawancin sun kasance mafi girma a cikin binciken 2. An gano Tyramine da phenylethylamine kawai a cikin rashin O. oeni.Wannan bincike yana ba da gudummawa ga sanin abubuwan tsaro na enterococci da ke da alaƙa da ruwan inabi.