Kayayyaki

Biotransformation na methyl-β-D-glucopyranoside zuwa mafi girma sarkar alkyl glucosides ta cell daure β-glucosidase na Pichia echellsii.

A cikin binciken da muke ciki, mun bincika amfani da Pichia echellsii gabaɗayan sel don haɗin sarkar alkyl glucosides.An yi amfani da Methyl-β-d-glucopyranoside (MG) a cikin amsawa tare da m alcohols, n-hexanol, n-octanol, n-decanol da n-dodecanol don haɗa nau'ikan alkyl glucosides.An inganta yanayin halayen farko na farko a sikelin 2.5 ml don haɗin octyl glucoside (OG) kuma sun kasance 8% abun ciki na ruwa, 100mM MG da 6h na lokacin amsawa kuma wannan ya haifar da ≈ 53% yawan amfanin ƙasa.Matsakaicin adadin transglucosylation/hydrolysis na 2.79 an samu a 100mM MG yana fifita yawan amfanin ƙasa.Dangane da ingantattun yanayi, an yi amfani da reactor a matakin 50 ml wanda ya haifar da ≈ 60% jujjuyawar MG zuwa OG.An ƙirƙiri hanyar chromatography mai sauƙi mai sauƙi mai ƙarfi don ƙididdige sarkar glucosides mafi girma ta amfani da mai gano fiddawa.Matsakaicin yawan amfanin ƙasa na 27% da 13% don decyl-, da dodecyl-β-d-glucopyranoside, bi da bi.