Kayayyaki

An haɓaka gwajin tabo mai sauƙi, wanda ke ba da damar ƙididdige microalbuminuria.An gudanar da kimantawa bisa ga ka'idodin ISO 15189. An hange fitsari a kan tubes acetate cellulose kuma an lalata su ta amfani da nau'in nau'in furotin mai mahimmanci (nigrosin, Coomassie Blue R-250, amido baki).An ƙididdige ƙarfin launi na tabo ta amfani da tashar Kodak Hoton 450. Analytical sensitivity na hanyar Coomassie Blue (18 mg / L) ya fi wannan don nigrosin (50 mg / L) ko amido baki (100 mg / L) ) tushen hanyoyin.Ƙididdigar tsaka-tsaki na tsaka-tsaki (CV) da tsaka-tsakin CV na Coomassie blue assay sun kasance, bi da bi, 8.4% da 9.7% (50 mg/L), da 3% da 4.5% (400 mg/L).Don nigrosin, waɗannan bayanan sun kasance, bi da bi, 8.4 da 9.4 (50 mg/L), da 3.4 da 6.4% (400 mg/L).Coomassie Blue ya nuna fifikon zaɓin ɗaure zuwa albumin.Hanyar da aka gano ta kasance mai layi tsakanin 20 zuwa 600 mg/l.An sami kyakkyawar alaƙa (r2 = 0.89) tsakanin tushen Coomassie Blue da ma'aunin rigakafi na rigakafi.Za a iya gano albumin na immuno-unreactive (wanda aka shirya ta hanyar maganin protease) ta gwajin tabo, wanda ke ba da fa'idar hanyar da gwaje-gwajen rigakafi.Ammonium sulphate hazo zai iya ƙara ƙayyadaddun ƙayyadaddun gwajin ta hanyar kawar da tasirin sarƙoƙi na haske. Hanyar da aka kwatanta tana da sauƙi kuma mai arha sosai, wanda ya sa ya dace da shirye-shiryen tantancewa, musamman a ƙasashen duniya na uku.