Kayayyaki

Masu karɓa na P2X3 na ATP-gated sune mahimmancin masu canzawa na abubuwan motsa jiki na nociceptive kuma kusan an bayyana su ta hanyar ƙananan ganglion neurons.A cikin linzamin kwamfuta trigeminal ganglion (TG), aikin mai karɓar P2X3 yana haɓaka ba zato ba tsammani ta hanyar pharmacological block na natriuretic peptide receptor-A (NPR-A), yana bayyana yiwuwar inhibitory peptides na endogenous natriuretic peptides a cikin nociception ta hanyar P2X3 masu karɓa.Rashin canji a cikin maganganun furotin na P2X3 yana nuna hadaddun tsarin daidaitawa wanda hanyoyin da za su rage aikin mai karɓar P2X3 ba su da tabbas.Don fayyace wannan tsari a cikin al'adun TG na linzamin kwamfuta, mun dakatar da siginar NPR-A tare da ko dai siRNA na agonist na ƙarshe na BNP, ko NPR-A. blocker anantin.Don haka, mun bincika canje-canje a cikin rarrabawar mai karɓa na P2X3 a cikin sashin raft membrane na lipid, yanayin phosphorylation su, da kuma aikin su tare da facin facin.Jinkirin farawa na rashin jin daɗi na P2X3 shine hanya ɗaya don haɓaka ayyukan anantin da aka haifar na P2X3.Anant a cikin aikace-aikacen ya haifar da sake rarraba mai karɓa na P2X3 na fifiko zuwa sashin raft na lipid kuma ya rage P2X3 serine phosphorylation, al'amura biyu waɗanda ba su dogara da juna ba.Wani mai hana cGMP-dogara protein kinase da siRNA-mediated knockdown na BNP ya kwaikwayi tasirin anantin.Mun nuna cewa a cikin linzamin kwamfuta trigeminal neurons endogenous BNP yana aiki akan masu karɓar NPR-A don ƙayyade ƙayyadaddun ciki na aikin mai karɓar P2X3.Tonic hanawa na P2X3 mai karɓar aikin ta hanyar BNP/NPR-A/PKG yana faruwa ta hanyar hanyoyi guda biyu: P2X3 serine phosphorylation da kuma sake rarraba mai karɓa zuwa sassan da ba raft membrane.Wannan sabon tsarin tsarin kula da mai karɓa zai iya zama manufa don nazarin gaba na gaba da nufin rage yawan aikin mai karɓa na P2X3 dysregulated a cikin ciwo mai tsanani.