Kayayyaki

Riboflavin, wanda kuma aka sani da bitamin B2, ana jigilar shi zuwa cikin membrane na halitta zuwa gabobin daban-daban ta tsarin sufuri.Riboflavin transporter RFVT3 an bayyana shi a cikin ƙananan hanji kuma an ba da shawarar don ganowa a cikin membranes apical na kwayoyin epithelial na hanji.A cikin wannan binciken, mun bincika aikin haɗin gwiwar RFVT3 a cikin shayarwar riboflavin ta amfani da kwayoyin T84 na intestinal epithelial da ƙananan hanji na linzamin kwamfuta.Kwayoyin T84 sun bayyana RFVT3 da kuma kiyaye jigilar riboflavin unidirectional daidai da sha na hanji.Apical [(3)H] shan riboflavin ya dogara da pH a cikin ƙwayoyin T84.Ragewar Na(+) bai shafe wannan ɗauka ba a pH 6.0 na apical, kodayake an rage shi sosai a pH 7.4.[(3)H] riboflavin da aka karɓa daga gefen apical na sel T84 an hana shi sosai ta hanyar RFVT3 mai zaɓin zaɓin methylene blue kuma ya ragu sosai ta hanyar canja wurin RFVT3-ƙananan-tsangwama RNA.A cikin ƙwayar gastrointestinal, an bayyana RFVT3 a cikin jejunum da ileum.Mouse jejunal da leal permeabilities na [(3)H] riboflavin an auna su ta hanyar rufaffiyar madauki kuma an rage su sosai da methylene blue.Waɗannan sakamakon suna ba da shawarar sosai cewa RFVT3 za ta kasance cikin aiki da hannu a cikin shayarwar riboflavin a cikin membranes apical na ƙwayoyin epithelial na hanji.