Kayayyaki

Ingantacciyar amfani da nitrogen a cikin shanu yana da mahimmanci don tabbatar da samar da shanu mai dorewa.Kamar yadda purines da pyrimidines (PP) suka zama wani yanki mai daraja na rumen nitrogen, ingantaccen fahimtar sha da tsaka-tsakin metabolism na PP yana da mahimmanci.Ayyukan na yanzu yana bayyana haɓakawa da kuma tabbatar da wata hanya mai mahimmanci da ƙayyadaddun hanya don ƙayyade lokaci guda na 20 purines (adenine, guanine, guanosine, inosine, 2'-deoxyguanosine, 2'-deoxyinosine, xanthine, hypoxanthine), pyrimidines (cytosine, thymine, uracil, cytidine, uridine, thymidine, 2'-deoxyuridine), da samfuran lalata su (uric acid, allantoin, β-alanine, β-ureidopropionic acid, β-aminoisobutyric acid) a cikin jini na jini na shanu.Babban aikin tushen fasaha na chromatography na ruwa haɗe zuwa electrospray ionization tandem mass spectrometry (LC-MS/MS) an haɗa shi tare da daidaitattun matakan daidaita matrix da madaidaicin madaidaicin madaidaicin ma'anar mahaɗan.An riga an gabatar da ƙididdigar ƙididdiga ta hanyar wani sabon tsari na riga-kafi wanda ya ƙunshi hazo ethanol, tacewa, evaporation da sake gyarawa.An bincika sigogi don rabuwa da ganowa yayin binciken LC-MS/MS.An tabbatar da cewa ta yin amfani da ƙirar log-calibration maimakon samfurin daidaitawa na layi ya haifar da ƙananan CV% kuma rashin gwajin dacewa ya nuna ci gaba mai gamsarwa.Hanyar ta ƙunshi jeri na maida hankali ga kowane metabolite bisa ga wancan a cikin ainihin samfuran, misali guanine: 0.10-5.0 μmol/L, da allantoin: 120-500 μmol/L.CV% na adadin da aka zaɓa ya kasance ƙasa da 25%.Hanyar tana da maimaituwa mai kyau (CV%≤25%) da daidaitattun tsaka-tsaki (CV%≤25%) da kyakkyawan farfadowa (91-107%).Duk metabolites sun nuna kyakkyawan kwanciyar hankali na dogon lokaci da kyakkyawan kwanciyar hankali a cikin gudu (CV%≤10%).An lura da digiri daban-daban na cikakken tasirin matrix a cikin plasma, fitsari da madara.Ƙaddamar da tasirin matrix dangi ya nuna cewa hanyar ta dace da kusan dukkanin PP metabolites da aka bincika a cikin plasma da aka zana daga wani artery da portal hepatic, hepatic da gastrosplenic veins kuma, tare da wasu 'yan kaɗan, kuma ga sauran nau'in irin su kaza, alade. mink, mutum da bera.