Kayayyaki

Kwayoyin Eukaryotic sun ƙunshi ma'auni mai ɗanɗano na mintuna kaɗan na deoxyribonucleoside triphosphates (dNTPs), wanda ya isa kawai na 'yan mintuna kaɗan na kwafin DNA.Duka rashi da ragi na dNTP guda ɗaya na iya haifar da haɓaka ƙimar maye gurbin, kuskuren gyaran DNA ko raguwar DNA mitochondrial.dNTPs yawanci ana ƙididdige su ta hanyar kididdigar enzymatic wanda haɗawar dATP na rediyoaktif (ko rediyoaktif dTTP a cikin tantancewar dATP) cikin takamaiman oligonucleotides na roba ta hanyar DNA polymerase ya yi daidai da tattarawar dNTP da ba a sani ba.Mun gano cewa Klenow DNA polymerase da aka saba amfani da shi na iya musanya madaidaicin ribonucleotide don dNTP wanda ba a san shi ba wanda ke jagorantar wasu lokuta zuwa babban kima na dNTPs.Yanzu mun bayyana yanayin tantancewa ga kowane dNTP waɗanda ke guje wa haɗa ribonucleotide.Don ƙididdigar dTTP da dATP ya isa don rage yawan ƙididdiga na Klenow enzyme da na dATP (ko dTTP);don dCTP da dGTP dole ne mu maye gurbin t he Klenow enzyme tare da ko dai Taq DNA polymerase ko Thermo Sequenase.Muna ba da shawarar cewa a cikin wasu rahotannin baya-bayan nan haɗin gwiwar ribonucleotide na iya haifar da ƙima mai yawa ga dGTP da dCTP.