Kayayyaki

4-Methylumbelliferyl β-D-glucopyranoside an yi amfani da shi azaman substrate: · a cikin glucosylceramidase β enzyme assay a cikin lysosome-enriched fractions daga primary hippocampal neurons · a β-glucosidase assay a lokacin yisti fermentation · to assay glucocerebrosidase 1 (GBAse1). aiki a cikin layin salula na macrophage (RAW)

4-Methylumbelliferyl β-D-glucopyranoside wani nau'in enzymatic na roba ne na glycosidase.An yi amfani dashi azaman substrate don β-glucosidase daga enterococci.

Mun bayar da rahoton cewa masu ba da gudummawar glycoside masu sauƙi na iya canza ma'aunin halayen glycosyltransferase-catalyzed, canza samuwar NDP-sugar daga endothermic zuwa wani tsari na exothermic.Don nuna amfanin wannan daidaitawar thermodynamic, muna haskaka haɗin glycosyltransferase-catalyzed na nucleotides na sukari guda 22 daga masu ba da gudummawar sukari mai sauƙi, da madaidaicin yanayin halittar nucleotides na sukari a matsayin ƙarfin tuƙi a cikin mahallin glycosyltransferase-catalyzed halayen ga ƙananan ƙwayoyin glycodiversification.Waɗannan masu ba da gudummawa mai sauƙi kuma sun ba da damar gwajin launi na gabaɗaya don glycosyltransfer, wanda ya dace da gano magunguna, injiniyan furotin da sauran mahimman binciken da suka dogara da sukari nucleotide.Wannan binciken kai tsaye ya kalubalanci ra'ayi na gaba ɗaya cewa NDP-sugars sune masu ba da gudummawar masu ciwon sukari 'mafi girma' lokacin da aka fitar da su daga mahallin nazarin halittu na al'ada.