Kayayyaki

Monogalactopyranosides na fluorescein da fluorescein methyl ester: kira, enzymatic hydrolysis ta biotnylated β-galactosidase, da ƙaddarar fassarar fassarar fassarar.

Fluorescein monoglycosides (d-galactopyranoside (FMG) da d-glucopyranoside) da methyl ester (MFMG) an shirya su daga acetobromoglucose / galactose da fluorescein methyl ester a cikin kyakkyawan amfanin gona.Gwaje-gwajen hydrolysis na enzymatic (ta amfani da biotinylated β-galactosidase) na abubuwan da aka samo na galacto an yi su kuma an ƙididdige sigogin motsi.An lura da haɓakar 15-20 sau na ƙarfin haske a lokacin hydrolysis.An lura da haɓakar madaidaiciyar haske a cikin ɗan gajeren lokaci da ƙarancin maida hankali na ƙasa, yana sa waɗannan mahadi masu amfani da bincike mai mahimmanci ga galactosidases.Girman darajar Michaelis-Menten akai (Km) na MFMG ya fi na FMG yana ba da shawara mai yuwuwar canjin yanayi na ma'auni na fluorogenic.Ƙimar km don β-Gal biotinylated tare da FMG ya yi ƙasa da wancan don enzyme na asali.Wannan abin lura yana nuna alaƙar daɗaɗɗen ƙwayar ƙwayar biotinylated idan aka kwatanta da enzyme na asali.An auna ma'auni na fassarar fassarar, don duka nau'ikan nau'ikan fluorogenic da duka samfuran, suna amfani da yanayin daidaita yanayin haske.Ƙididdigar rarraba fassarar fassarar don abubuwan da ake amfani da su na fluorogenic da samfurori na enzymatic hydrolysis an auna su su kasance iri ɗaya, a cikin kewayon 3.5-4.5 × 10-10 m2 s-1.Don haka haɓakawa ko jinkirin motsin motsin enzymatic saboda bambanci a cikin motsin fassarar substrate da samfur bai bayyana ba.