Kayayyaki

Mun ba da rahoton kwanan nan cewa pyrene nucleotide an fi dacewa an saka shi a gaban wani wuri mara kyau, 3'-T na dimer na thymine, kuma mafi yawan sansanonin da ba a lalacewa ta hanyar yisti DNA polymerase eta (pol eta).Saboda pyrene kwayoyin halittar da ba na polar ba ne ba tare da ikon haɗin kai na H ba, ƙimar da ba a saba gani ba na shigar dPMP ana danganta shi da mafi girman ikon tari na tushe, kuma yana nuna mahimmancin tari tushe cikin zaɓin nucleotides ta hanyar pol eta.Don bincika rawar H-bonding da tushe guda biyu na geometry a cikin zaɓin nucleotides ta hanyar pol eta, mun ƙaddamar da ingantattun ingantattun abubuwan da aka gyara na nucleotides 2,6-diaminopurine, 2-aminopurine, 6-chloropurine, da inosine waɗanda zasu iya. yi daban-daban adadin H-bonds tare da samfurin tushe dangane da tushe biyu lissafi.Watson-Crick base pairing ya bayyana yana taka muhimmiyar rawa a cikin zaɓin analogues na nucleotide don sakawa sabanin C da T kamar yadda aka tabbatar ta hanyar raguwar ingancin shigar dangi tare da raguwar adadin Watson-Crick H-bonds da karuwa a cikin yawan hulɗar masu ba da gudummawa da karɓa-karɓa.Zaɓin shigar da nucleotide ya fi girma a gaban 5'-T fiye da 3'-T na dimer thymine, daidai da aikin da ya gabata yana nuna cewa 5'-T yana riƙe da ƙarfi fiye da 3'-T.Bugu da ƙari, shigar da A akasin duka Ts na dimer ya bayyana ana yin sulhu ta hanyar haɗin tushe na Watson-Crick kuma ba ta hanyar haɗin gwiwar tushe na Hoogsteen ba dangane da ingantaccen shigar da kusan iri ɗaya na A da 7-deaza-A, ƙarshen wanda ba shi da H- iya aiki akan N7.Ingantattun ingantattun alaƙa don shigar da nucleotides waɗanda zasu iya samar da nau'ikan nau'ikan tushe na Watson-Crick daidai da waɗanda ke guntun Klenow, yayin da guntun Klenow ya fi nuna wariya ga rashin daidaituwa, daidai da zaɓin siffarsa mafi girma.Waɗannan sakamakon suna nuna mahimmancin nau'ikan lissafi na H-bonding da Watson-Crick tushe biyu a cikin zaɓin nucleotides ta duka biyun pol eta da guntun Klen ow, da ƙaramin aikin zaɓin siffa a cikin shigar ta hanyar pol eta saboda ƙarin buɗewa da ƙasa. ƙuntataccen wurin aiki.